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中國精品科技期刊2020

殼聚糖涂覆磁性納米顆粒對纖維素酶的固定化

王琳 劉容旭 劉丹怡 王語聰 韓建春

王琳,劉容旭,劉丹怡,等. 殼聚糖涂覆磁性納米顆粒對纖維素酶的固定化[J]. 食品工業科技,2022,43(16):74?80. doi:  10.13386/j.issn1002-0306.2021110183
引用本文: 王琳,劉容旭,劉丹怡,等. 殼聚糖涂覆磁性納米顆粒對纖維素酶的固定化[J]. 食品工業科技,2022,43(16):74?80. doi:  10.13386/j.issn1002-0306.2021110183
WANG Lin, LIU Rongxu, LIU Danyi, et al. Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles[J]. Science and Technology of Food Industry, 2022, 43(16): 74?80. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021110183
Citation: WANG Lin, LIU Rongxu, LIU Danyi, et al. Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles[J]. Science and Technology of Food Industry, 2022, 43(16): 74?80. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021110183

殼聚糖涂覆磁性納米顆粒對纖維素酶的固定化

doi: 10.13386/j.issn1002-0306.2021110183
詳細信息
    作者簡介:

    王琳(1991?),女,碩士研究生,研究方向:農產品加工及貯藏,E-mail:1360576651@qq.com

    通訊作者:

    韓建春(1973?),男,博士,教授,研究方向:農產品加工及貯藏,E-mail:hanjianchun@hotmail.com

  • 中圖分類號: TS201.2

Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles

  • 摘要: 設計具有高穩定性、選擇性的酶-載體復合物是固定化酶領域的研究重點。本研究以環氧氯丙烷作為表面活性劑,通過沉淀法制備磁性納米顆粒并涂覆殼聚糖,用以固定化纖維素酶。通過SEM掃描電鏡、VSM磁強計、FTIR紅外光譜對 Fe3O4-殼聚糖磁性納米顆粒進行表征,并研究其固定化纖維酶的表征及酶學性質。結果表明,制備的磁性納米顆粒晶形完整,纖維素酶有效固定在Fe3O4-殼聚糖載體表面;固定化纖維素酶比游離纖維素酶具有更好的酸堿穩定性和熱穩定性。固定化纖維素酶在pH 2~9范圍內均有較好的活性,并且置于60和70 ℃條件下4 h后,仍然能保持將近50%的活性,經10次循環利用后,固定化纖維素酶仍然保持在52.6%的活性,說明Fe3O4-殼聚糖可作為固定纖維素酶的有效載體,為固定化酶的進一步應用提供了參考。
  • 圖  1  Fe3O4(a、b)、Fe3O4-殼聚糖(c)的掃描電鏡圖

    Figure  1.  SEM images of Fe3O4 (a, b), Fe3O4-chitosan magnetic nanoparticles(c)

    圖  2  Fe3O4磁性納米顆粒、Fe3O4-殼聚糖磁性納米顆粒的磁滯回線

    Figure  2.  Magnetization hysteresis loops for Fe3O4, Fe3O4-chitosan magnetic nanparticles

    圖  3  Fe3O4,Fe3O4-殼聚糖磁性納米顆粒FTIR譜圖

    Figure  3.  FTIR spectra of Fe3O4, Fe3O4-chitosan magnetic nanparticles

    圖  4  Fe3O4-殼聚糖磁性納米顆粒循環利用次數對纖維素酶固載量的影響

    Figure  4.  Effect of recycling times of Fe3O4-chitosan magnetic nanoparticles on the amount of cellulase immobilized

    圖  5  Fe3O4-殼聚糖磁性納米顆粒、纖維素酶復合體系的FTIR光譜圖

    Figure  5.  FTIR spectra of Fe3O4-chitosan magnetic nanoparticles, cellulase complex system

    圖  6  Fe3O4-殼聚糖磁性納米顆粒(a)、纖維素酶復合體系(b)在水溶液中的分散穩定性

    Figure  6.  Dispersion stability of Fe3O4-chitosan magnetic nanoparticles(a),cellulase complex system(b) in water solution

    圖  7  磁場輔助分離水溶液中固定化纖維素酶的Fe3O4-殼聚糖納米顆粒的示意圖

    Figure  7.  Schematic diagram of magnetic field assisted separation of Fe3O4-chitosan nanoparticles immobilized with cellulase in aqueous solution

    圖  8  pH對游離纖維素酶和固定化纖維素酶的影響

    Figure  8.  Effects of pH on free cellulase and immobilized cellulase

    圖  9  游離纖維素酶和固定化纖維素酶的溫度穩定性

    Figure  9.  Thermal stability of free cellulase and immobilized cellulase

    圖  10  循環利用次數對纖維素酶活性的影響

    Figure  10.  Effect of recycle number on cellulase activity

    表  1  Fe3O4、Fe3O4-殼聚糖磁性納米顆粒、纖維素酶復合體系的粒徑和電勢

    Table  1.   Potential and Particle size of Fe3O4, Fe3O4-chitosan magnetic nanoparticles, cellulase complex system

    類型粒徑(nm)電勢(mV)
    Fe3O431.4±1.43?32.2±0.25
    Fe3O4-殼聚糖42.3±0.8?15.2±0.38
    Fe3O4-殼聚糖-纖維素酶88.2±0.40.36±0.12
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